Lipidomics reveals multiple stressor effects (temperature × mitochondrial toxicant) in the zebrafish embryo toxicity test

Aquatic organisms are exposed to multiple stressors in the environment, including contaminants and rising temperatures due climate change. The objective of this study was characterize effect increased temperature on chemical-induced toxicity lipid profiles during embryonic development hatch fish. This is important because many environmental chemicals modulate cellular metabolism lipids, both which play integral roles for normal development. As such, we employed zebrafish embryo test stressor exposures, using mitochondrial toxicant 2,4-Dinitrophenol (DNP; 6–30 μM) conjunction with different treatments (28 °C 33 °C). We found a positive relationship between lethality at lower DNP concentrations, suggesting stress can increase sensitivity. Next, used LC-MS/MS lipidomics following exposure sublethal combinations. It determined that augmented DNP-induced effects lipidome, upregulation bioactive lipids involved apoptosis (e.g., ceramides). These data reveal potential implications change sensitivity pollution demonstrate utility metabolic pathways underlying toxicity. Data such as these expected advance adverse outcome by establishing networks include intermediate responses.